Tohoku Univ. Developed by Dr. Masanobu Satake, Tohoku University in 1998. A targeting vector containing pgk-neo cassette was transfered into ES cells to relpace exons 4. AML1 KO, Runx1 KO mice AML1 KO, Runx1 KO mice Cancer Research 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Oncogene, 17, 2287-2293 (1998).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は本件リソースを利用した研究成果の公表にあたって謝辞の表明を必要とする。共同研究として用い、寄託後２年間は論文や学会での発表の際は共著とする。寄託者の研究と競合する用途を避けるために、予定の研究概要を寄託者に知らせ、使用許可を得る。本件リソースの特許権ならびに知的所有権は寄託者が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合には事前に寄託者と別途協議する。 C (3-6 months) mouse Runx1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Cell Biology Research B6.129S4-Runx1<tm1Sata>. Runx1, runt related transcription factor 1 (also called AML1) encodes the DNA binding subunit of the heterodimeric transcription factor. Runx1 is one of the most frequent targets of chromosome translocation associated with several subtypes of human acute myeloid or lymphoblastic leukemia. Mice homozygous for the Runx1 null mutantion die embryonic day 12.5 due to massive hemorrhage in the central nervous system. In fetal livers of mutant embryos, no hematopoieteic cells are generated. C（3〜6か月） B6.129S4-Runx1<tm1Sata>/SataRbrc Masanobu SATAKE The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 17, 2287-2293 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use <br>of it from the DEPOSITOR to avoid possible conflicts with DEPOSITOR.<br>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a <br>citation of the following literature designated by the DEPOSITOR is requested.<br>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE,<br>an acknowledgment to the DEPOSITOR is requested.<br> The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications <br>resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.<br>The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) RBRC01450 <a href='https://brc.riken.jp/mus/pcr01450'>Genotyping protocol -PCR-</a> ホモで不妊／死亡 佐竹　正延 Runx1 KO mice 作製後、B6/Jに25代バッククロスした。 国立大学法人東北大学 AML1 KO AML1 KO true